A novel pH probe based on a rhodamine–rhodamine platform

We developed a rhodamine–rhodanine-based pH probe with p K a = 4.85 in buffer solution. The fluorescence intensity exhibited strong pH-dependent performance and responded linearly to minor pH fluctuations within the range of 4.2–5.2. In addition, the fluorescence microscopic images suggested this pr...

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Bibliographic Details
Published inRSC advances Vol. 4; no. 92; pp. 50318 - 50324
Main Authors Zhao, Xuan-Xuan, Chen, Xin-Peng, Shen, Shi-Li, Li, Dong-Peng, Zhou, Shuai, Zhou, Ze-Quan, Xiao, Yu-Hao, Xi, Gang, Miao, Jun-Ying, Zhao, Bao-Xiang
Format Journal Article
LanguageEnglish
Published 01.01.2014
Subjects
Buffer solutions
Environmental monitoring
Fluctuation
Fluorescence
Lysosomes
Platforms
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Summary:We developed a rhodamine–rhodanine-based pH probe with p K a = 4.85 in buffer solution. The fluorescence intensity exhibited strong pH-dependent performance and responded linearly to minor pH fluctuations within the range of 4.2–5.2. In addition, the fluorescence microscopic images suggested this probe had excellent cell membrane permeability and could image weak acid pH changes of lysosomes in live cells without auto-fluorescence and interference from the complex intracellular environment. The results demonstrated that the probe had great potential in monitoring H + in vitro and in living cells.
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ISSN:2046-2069
2046-2069
DOI:10.1039/C4RA07555B