New redox and conducting polymer modified electrodes for cholesterol biosensing

New enzyme biosensors for cholesterol detection based on a recently developed transduction platform constituted of a GCE modified with polymer redox mediator poly(methylene blue) (PMB) and conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) were for the first time prepared and evaluated. The...

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Bibliographic Details
Published inAnalytical methods Vol. 5; no. 5; pp. 1199 - 1204
Main Authors Kakhki, Somayeh, Barsan, Madalina M., Shams, Esmaeil, Brett, Christopher M. A.
Format Journal Article
LanguageEnglish
Published 01.01.2013
Subjects
Biosensors
Cholesterol
Cholesterol oxidase
Conducting polymers
Electrical measurements
Electrodes
Enzymes
Monitoring
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Summary:New enzyme biosensors for cholesterol detection based on a recently developed transduction platform constituted of a GCE modified with polymer redox mediator poly(methylene blue) (PMB) and conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) were for the first time prepared and evaluated. The enzyme cholesterol oxidase (ChOx) was immobilized by simple adsorption, the ChOx/PEDOT/PMB/GCE biosensor being applied for amperometric determination of cholesterol by monitoring the peroxide reduction produced by the enzymatic reaction at -0.4 V vs.SCE. Amperometric studies at fixed potential reveal that almost interference-free cholesterol determination can be achieved at the newly developed biosensor in a range between 10 and 220 mu M with a sensitivity of 79.0 mu A cm super(-2) mM super(-1) and a detection limit of 1.6 mu M. The recovery and storage stability of the biosensor were evaluated and the biosensor was applied to cholesterol detection in whole cow milk and chicken egg yolk.
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ISSN:1759-9660
1759-9679
DOI:10.1039/c3ay26409b