In vitro kinetics of the oxidative reactivity of nitrate and nitrite in the rat erythrocyte

Isolated rat erythrocytes were incubated in the presence of nitrate and nitrite. Glucose, lactate, reduced glutathione, methaemoglobin, malondialdehyde and Na+/K+ membrane exchange were investigated. Nitrite induced a strong methaemoglobinaemia and a net depletion of reduced glutathione in the intra...

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Bibliographic Details
Published inFood additives and contaminants Vol. 7 Suppl 1; p. S145
Main Authors Batina, P, Fritsch, P, de Saint Blanquat, G, Mitjavila, M T
Format Journal Article
LanguageEnglish
Published England 1990
Subjects
Animals
Cell Membrane - drug effects
Cell Membrane - metabolism
Cells, Cultured
Erythrocytes - drug effects
Erythrocytes - metabolism
Glucose - metabolism
Kinetics
Lactates - biosynthesis
Male
Nitrates - pharmacology
Nitrites - pharmacology
Oxidation-Reduction
Potassium - metabolism
Rats
Rats, Inbred Strains
Sodium - metabolism
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Summary:Isolated rat erythrocytes were incubated in the presence of nitrate and nitrite. Glucose, lactate, reduced glutathione, methaemoglobin, malondialdehyde and Na+/K+ membrane exchange were investigated. Nitrite induced a strong methaemoglobinaemia and a net depletion of reduced glutathione in the intracellular medium associated with membrane lipid peroxidation. This oxidative reactivity induced by nitrate and nitrite altered the cell's ionic flux.
ISSN:0265-203X
DOI:10.1080/02652039009373868