DNA polymerase β mRNA and protein expression in Xiphophorus fish

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 138; no. 3; pp. 325 - 334
• Main Authors: Heater, Sheila J., Oehlers, Leon P., Rains, J. Douglas, Walter, Ronald B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2004
• Subjects: Animals; Base excision repair; Base Sequence; Cyprinodontiformes - genetics; Cyprinodontiformes - metabolism; DNA Polymerase beta - genetics; DNA Polymerase beta - metabolism; DNA polymerase β; DNA, Complementary - genetics; Fish; Gene expression; Gene Expression Profiling; Interspecies hybrids; Melanoma; Molecular Sequence Data; mRNA expression; Real time RT-PCR; RNA, Messenger - genetics; RNA, Messenger - metabolism; Western blot; Western blot; Interspecies hybrids; Real time RT-PCR; Melanoma; Fish; DNA polymerase β; Gene expression; Base excision repair; mRNA expression
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cca.2004.09.002

Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase β protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Polβ expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis.