Priming of the respiratory burst in neutrophils exposed to a combination of weak constant and alternating low-frequency magnetic fields in vitro

• Published in: Biophysics (Oxford) Vol. 61; no. 3; pp. 429 - 434
• Main Authors: Novikov, V. V., Yablokova, E. V., Fesenko, E. E.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.05.2016; Springer Nature B.V
• Subjects: Biological and Medical Physics; Biophysics; Cell Biophysics; Luminescence; Magnetic fields; Oxygen; Physics; Physics and Astronomy; Rodents; magnetic field; neutrophils; chemiluminescence; reactive oxygen species
• ISSN: 0006-3509; 1555-6654
• DOI: 10.1134/S000635091603012X

An hour-long exposure of peritoneal neutrophils of mice to a combination of a weak constant magnetic field (42 μT) and low-frequency alternating magnetic fields collinear to the weak constant magnetic field (frequencies 1, 4.4, and 16.5 Hz, total amplitude 0.86 μT) at physiological temperatures promoted a significant increase in chemiluminescence of cells in response to subsequent exposure to low concentrations of respiratory burst activators (formylated peptide N-formyl-Met–Leu–Phe or phorbol ester phorbol-12-myristate-13-acetate) in the presence of luminol. The response of human neutrophils isolated from peripheral blood to the pretreatment with combined magnetic fields followed by exposure to the activator N-formyl-Met–Leu–Phe was similar to the response of mouse neutrophils.