Membrane change in the early stage of D-galactosamine induced hepatic injury

• Published in: Kanzo Vol. 19; no. 4; pp. 339 - 345
• Main Authors: NODA, Ken-ichi, OKITA, Kiwamu, TAKENAMI, Toshihiko, KODAMA, Takahiro, HARADA, Toshihiro, TAKEMOTO, Tadayoshi
• Format: Journal Article
• Language: English; Japanese
• Published: The Japan Society of Hepatology 1978
• Subjects: 5'-nucleotidase; D-galactosamine
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.19.339

The possible role of liver cell plasma membrane (cell membrane) through the induction of hepatic injury by D-galactosamine (gal) was studied from the view points of 14C-amino acid pooling in the cell membrane and synthesis of the cell membrane protein. The specificity of the cell membrane which was collected by the method of Neville was examined by 5'-nucleotidase activity and electromicroscopic findings. The membrane protein synthesis was determined by the count of 14C(dpm) in each sections of gel, which were separated by means of 0.1% SDS-polyacrylamide gel electrophoresis. Amino acid pool and synthesis of the cell membrane protein decreased up to 2 hours after gal (400mg/kg body weight i.p.) injection. However, these findings mentioned above, was never found in the cell membrane from the rats which were treated with gal and uridine (1.2mg/g body weight i.p.). Therefore, we want to point out the remarkable decrease of amino acid pool and synthesis of the protein in the cell membrane from gal treated rats within a short time when necrosis was never found. Then, the cell membrane may also play an important role for the induction of liver cell necrosis.