Transducin-activated cGMP-specific phosphodiesterase of external segments of bovine retinal rods: The influence of magnesium ions

The kinetic behavior of phosphodiesterase activated by transducin in a complex with a non-hydrolyzable GTP analogue (guanosine-5′-O-thiotriphosphate) was studied by the pH-metric method in preparations of light-adapted external segments of bovine retinal rods in a range of magnesium ion concentratio...

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Published inBiophysics (Oxford) Vol. 61; no. 6; pp. 877 - 879
Main Authors Petrukhin, O. V., Orlova, T. G., Nezvetsky, A. R., Orlov, N. Ya
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.11.2016
Springer Nature B.V
Subjects
Biological and Medical Physics
Biophysics
Ions
Magnesium
Molecular Biophysics
Physics
Physics and Astronomy
Proteins
cGMP-specific phosphodiesterase
regulation by Mg
phototransduction system
and Ca
transducin
ions
external segments of retinal rods
G-proteins
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Summary:The kinetic behavior of phosphodiesterase activated by transducin in a complex with a non-hydrolyzable GTP analogue (guanosine-5′-O-thiotriphosphate) was studied by the pH-metric method in preparations of light-adapted external segments of bovine retinal rods in a range of magnesium ion concentrations from 0.4 to 20 mM. These results indicate that when using the reaction media containing 10–15 mM Mg 2+ ions, introduction of 2–4 mM ethylene glycol tetraacetic acid (a chelator of calcium ions) in the reaction medium induces only relatively small changes in the concentration of free magnesium ions that are not able to significantly influence the phosphodiesterase activity.
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ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350916050237