Deveploping Microsatellite Markers for Insect Population Structure: Complex Variation in a Checkerspot Butterfly
We isolated and characterized two microsatellite markers from the genome of the endangered checkerspot butterfly Melitaea cinxia L. In Finland, this species only survives on the Åland islands, where it exhibits a highly fragmented metapopulation structure on small meadows. Four alleles were observed...
Saved in:
Published in | Hereditas Vol. 123; no. 3; pp. 295 - 300 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Munksgaard International Publishers
01.04.1995
|
Online Access | Get full text |
Cover
Loading…
Summary: | We isolated and characterized two microsatellite markers from the genome of the endangered checkerspot butterfly Melitaea cinxia L. In Finland, this species only survives on the Åland islands, where it exhibits a highly fragmented metapopulation structure on small meadows. Four alleles were observed at the locus CINXI and nine at CINX4; the total gene diversities at the two loci were Hh= 0.34 and 0.80, respectively. A pilot survey showed moderate gene frequency differentiation among meadows (local populations; FLM= 0.1) and among metapopulations c. 30 km apart (FMT= 0.2). Contrary to prior expectation, distinct feeding larval groups collected in the spring did not represent offspring of single females. There was a conspicuous excess of homozygotes within local populations (FIL= 0.35), which can hardly be attributed to population structure alone; this urges caution in straightforward interpretation of microsatellite phenotype data. |
---|---|
Bibliography: | ArticleID:HRD2295 istex:5446152D67825F91BBCE27203550040172E4FCD4 ark:/67375/WNG-MJFP382N-F |
ISSN: | 0018-0661 1601-5223 |
DOI: | 10.1111/j.1601-5223.1995.00295.x |