Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 25; no. 5; pp. 575 - 577
• Main Author: 李新宇 李中国 邱良秀 赵长松 胡竹林
• Format: Journal Article
• Language: English
• Published: China Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2005
• Subjects: Animals; Cell Proliferation - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Corneal - cytology; Epithelium, Corneal - cytology; Nerve Growth Factor - pharmacology; Rabbits; 上皮细胞; 动物实验; 神经生长因子; 细胞增殖; 角膜损伤; corneal endothelial cells; corneal epithelial cells; proliferation; nerve growth factor
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/bf02896022

Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.