Quantitative 1H NMR with global spectral deconvolution approach for quality assessment of natural and cultured Cordyceps sinensis

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 235; p. 115603
• Main Authors: Liu, Qian-Bao, Liu, Jiazheng, Lu, Jing-Guang, Yang, Ming-Rong, Zhang, Wei, Li, Wen-Jia, Qian, Zheng-Ming, Jiang, Zhi-Hong, Bai, Li-Ping
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.10.2023
• Subjects: Cordyceps sinensis (C. sinensis); global spectral deconvolution (GSD); qNMR; TOF-SIMS; UHPLC-QTOF-MS; global spectral deconvolution (GSD); Cordyceps sinensis (C. sinensis); UHPLC-QTOF-MS; TOF-SIMS; qNMR
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2023.115603

Cordyceps sinensis is a precious medicinal food which has been successfully cultivated indoors. It remains to be investigated for a simultaneous comparison on aqueous components of natural and cultivated samples. Herein, an approach of quantitative nuclear magnetic resonance (qNMR) analysis combined with global spectral deconvolution (GSD) was established for simultaneous quantification of 26 aqueous components in C. sinensis. Processed by GSD, the distorted baselines of 1H-NMR spectra were greatly improved, and overlapped signals were also well separated so as to achieve accurate identification and quantitation of components in C. sinensis. Method validation by UHPLC-QTOF-MS and TOF-SIMS analysis revealed that qNMR combined with GSD is a reliable approach for simultaneous quantification of multiple components including characteristic markers of glutamine, GABA and trehalose in authentic and fake C. sinensis. The well-established qNMR approach can be used for quality assessment of natural and cultivated C. sinensis as well as differentiation from fake ones. [Display omitted] •qNMR analysis combined with GSD was firstly developed to analyze C. sinensis.•Glutamine, GABA and trehalose were proposed as markers of genuine and fake C. sinensis.•The method of qNMR analysis combined with GSD was validated by UHPLC-QTOF-MS.•The chemical distributions of markers were intuitively revealed by TOF-SIMS.•qNMR analysis combined with GSD was demonstrated as a reliable approach to comprehensively evaluate the quality of TCMs.