Development of luminol-N-hydroxyphthalimide chemiluminescence system for highly selective and sensitive detection of superoxide dismutase, uric acid and Co2

• Published in: Biosensors & bioelectronics Vol. 99; pp. 519 - 524
• Main Authors: Saqib, Muhammad, Qi, Liming, Hui, Pan, Nsabimana, Anaclet, Halawa, Mohamed Ibrahim, Zhang, Wei, Xu, Guobao
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.01.2018
• Subjects: Chemiluminescence; Cobalt ions; Luminol; N-Hydroxyphthalimide; Superoxide dismutase; Uric acid; Superoxide dismutase; Chemiluminescence; Uric acid; Cobalt ions; N-Hydroxyphthalimide; Luminol
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.08.028

N-hydroxyphthalimide (NHPI), a well known reagent in organic synthesis and biochemical applications, has been developed as a stable and efficient chemiluminescence coreactant for the first time. It reacts with luminol much faster than N-hydroxysuccinimide, eliminating the need of a prereaction coil used in N-hydroxysuccinimide system. Without using prereaction coil, the chemiluminescence peak intensities of luminol-NHPI system are about 102 and 26 times greater than that of luminol-N-hydroxysuccinimide system and classical luminol-hydrogen peroxide system, respectively. The luminol-NHPI system achieves the highly sensitive detection of luminol (LOD = 70pM) and NHPI (LOD = 910nM). Based on their excellent quenching efficiencies, superoxide dismutase and uric acid are sensitively detected with LODs of 3ng/mL and 10pM, respectively. Co2+ is also detected a LOD of 30pM by its remarkable enhancing effect. Noteworthily, our method is at least 4 orders of magnitude more sensitive than previously reported uric acid detection methods, and can detect uric acid in human urine and Co2+ in tap and lake water real samples with excellent recoveries in the range of 96.35–102.70%. This luminol-NHPI system can be an important candidate for biochemical, clinical and environmental analysis. •Chemiluminescence of luminol-NHPI has been reported for the first time.•Superoxide dismutase and uric acid can remarkably quench the chemiluminescence.•Co2+ can dramatically enhance the chemiluminescence of luminol-NHPI.•Highly sensitive detection of superoxide dismutase, uric acid and Co2+ is achieved.•It can successfully detect uric acid in human urine and Co2+ in water real samples after dilution.