Study of Mechanisms of Relaxation in Rat Blood Vessels Differing by Density and Innervation Type

• Published in: Journal of evolutionary biochemistry and physiology Vol. 40; no. 3; pp. 321 - 330
• Main Authors: Leontieva, G. R., Leontiev, V. G., Krivchenko, A. N.
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01.05.2004
• Subjects: Blood vessels; Proteins; Rodents
• ISSN: 0022-0930; 1608-3202
• DOI: 10.1023/B:JOEY.0000042637.12502.19

The work deals with a study of physiological significance of relaxation mechanisms initiated by stimulation of β-adrenoreceptors and NO-dependent pathways as well as of participation of cyclic nucleotide signal pathways in dilatation of rat blood vessels with different density and type of nervous regulation: of thoracic aorta with poorly developed adrenergic vasoconstrictional innervation, of tail artery with well-developed adrenergic vasoconstrictional innervation, and of portal vein with adrenergic and cholinergic vasoconstrictional innervation. Isometric contraction of 1-mm wide vascular rings produced by electrostimulation or action of exogenous selective antagonists (phenylephrine for α^sub 1^-adrenoreceptors of all three vessels and 5-methylformetide for muscarinic cholinergic receptors of portal vein) was recorded using a laboratory-made highly sensitive device. From relaxants, isoproterenol, sodium nitroprusside, forskolin, and isobutylmethylxanthine were used. As a result of the performed study with use of substances affecting various chains of processes providing relaxation of smooth muscle contractile apparatus of blood vessels with different degree of development of nervous regulation, we have obtained data that indicate heterogeneity of intracellular mechanisms of transmission of external signal realizing the contractionndash;relaxation cycle in these vessels.[PUBLICATION ABSTRACT]