Desorption and reusability of adsorbent Diaion HP-20 for the recovery of paclitaxel from culture supernatants of Taxus chinensis

• Published in: Biotechnology and bioprocess engineering Vol. 30; no. 3; pp. 573 - 587
• Main Authors: Kang, Yeji, Kim, Jin-Hyun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.06.2025; 한국생물공학회
• Subjects: Adsorbents; Adsorption; Air pollution; Cancer therapies; Cavitation; Cell culture; Desorption; Disease; Efficiency; Electron microscopy; Fourier transforms; Infrared analysis; Infrared spectroscopy; Paclitaxel; Porous resins; Recovery; Scanning electron microscopy; Side effects; Solvents; Spectrum analysis; Taxus chinensis; Temperature; Ultrasonic imaging; X-ray diffraction; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-025-00200-2

In this study, an ultrasonic desorption process was developed for the efficient recovery of paclitaxel from Taxus chinensis culture supernatants using a commercial adsorbent, Diaion HP-20. In addition, the reusability of the adsorbent was investigated. The optimal ultrasonic power, operating time, adsorbent/solvent ratio, temperature, solvent type, and concentration for ultrasonic desorption were 180 W, 20 min, 1:30 w/v, 25 °C, methanol, and 100%, respectively. Furthermore, all of the paclitaxel could be recovered after three desorption cycles. The high bubble collapse intensity of acoustic cavitation played a more decisive role than the high bubble density of hydrodynamic cavitation. Diaion HP-20 maintained a high adsorption efficiency of over 95% even after five regenerations, and the stability of the adsorbent was confirmed through electron microscopy, scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy analyses.