Whole Genome Analysis and Elucidation of Docosahexaenoic Acid (DHA) Biosynthetic Pathway in Aurantiochytrium sp. SW1

• Published in: Gene Vol. 846; p. 146850
• Main Authors: Prabhakaran, Pranesha, Raethong, Nachon, Nazir, Yusuf, Halim, Hafiy, Yang, Wu, Vongsangnak, Wanwipa, Abdul Hamid, Aidil, Song, Yuanda
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 20.12.2022
• Subjects: desaturase/elongases pathway; fatty acid synthase (FAS) pathway; fatty acids synthesis; polyketide synthase (PKS)-like PUFA pathway; polyunsaturated fatty acid (PUFA); Thraustochytrids; DH; PUFA; polyunsaturated fatty acid (PUFA); KR; KS; fatty acids synthesis; polyketide synthase (PKS)-like PUFA pathway; ER; ACP; AT; CoA; desaturase/elongases pathway; PPT; DNA; PKS; FAS; Thraustochytrids; DHA; SMART; fatty acid synthase (FAS) pathway
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2022.146850

•A hybrid system incorporating elements of FAS, Type I PKS and Type II PKS systems involved in the biosynthetic pathways of fatty acids in Aurantiochytrium sp. SW1.•Several of the enzymatic domains embedded in the multi-domain subunits of PUFA synthases preferential for PUFA, especially DHA biosynthesis.•Up to ten conserved multi-tandem ACP domains present in PUFA synthase subunit A (pfaA), contributing in high DHA content. Aurantiochytrium sp., a fungoid marine protist that belongs to Stramenophila has proven its potential in the production of polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acids (DHA). In this study, genomic characterisation of a potential producer for commercial production of DHA, Aurantiochytrium sp. SW1 has been carried out via whole genome sequencing analysis. The genome size of this strain is 60.89 Mb, with a total of 11,588 protein-coding genes. Among these, 9,127 genes could be functionally annotated into a total of 7,248 (62.5%) from UniProt, 6,554 (56.6%) from KEGG and 8,643 (74.6%) genes from eggNOG protein database. The highest proportion of genes belongs to the protein family of metabolism were further assigned into 11 metabolic categories. The highest number of genes belonging to lipid metabolism (321 genes) followed by carbohydrate metabolism (290 genes), metabolism of cofactors and vitamins (197 genes) and amino acid metabolism (188 genes). Further analysis into the biosynthetic pathway for DHA showed evidence of all genes involved in PKS (polyketide synthase)-like PUFA synthase pathway and incomplete fatty acid synthase-elongase/desaturase pathway. Analysis of PUFA synthase showed the presence of up to ten tandem acyl carrier protein (ACP) domains which might have contributed to high DHA production in this organism. In addition, a hybrid system incorporating elements of FAS, Type I PKS and Type II PKS systems were found to be involved in the biosynthetic pathways of fatty acids in Aurantiochytrium sp. SW1. This study delivers an important reference for future research to enhance the lipid, especially DHA production in Aurantiochytrium sp, SW1 and establishment of this strain as an oleaginous thraustochytrid model.