Design of a surrogate for high throughput screening of fatty aldehyde reductase engineering

We designed and synthesized a fatty aldehyde surrogate containing a formyl thioester group, which can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay. We demonstrated its successful applica...

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Published inChemical communications (Cambridge, England) Vol. 57; no. 98; pp. 13373 - 13376
Main Authors Worakaensai, Suphanida, Kluaiphanngam, Surayut, Wet-osot, Sirawit, Charoenwattanasatien, Ratana, Ngivprom, Utumporn, Duangkamol, Chuthamat, Kamkaew, Anyanee, Lai, Rung-Yi
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 09.12.2021
Subjects
Aldehyde Oxidoreductases - chemistry
Aldehyde Oxidoreductases - metabolism
Aldehydes
Aldehydes - chemistry
Chemical Engineering - methods
Fatty Acids - chemistry
High-Throughput Screening Assays - methods
Reductases
Screening
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Summary:We designed and synthesized a fatty aldehyde surrogate containing a formyl thioester group, which can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay. We demonstrated its successful application in the high throughput screening of FALR engineering. A fatty aldehyde surrogate containing a formyl thioester group can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay for screening applications.
Bibliography:10.1039/d1cc05472d
Electronic supplementary information (ESI) available. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc05472d