Gold nanoclusters – 2D MoS2 nano couple: An immunosensor for the fluorescence turn-on detection of heart failure Biomarker Brain Natriuretic Peptide (BNP)

• Published in: Journal of photochemistry and photobiology. A, Chemistry. Vol. 452; p. 115604
• Main Authors: Madanan Anju, S., Abraham Merin, K., Varghese, Susan, Ibrahim Shkhair, Ali, Rajeevan, Greeshma, Indongo, Geneva, George, Sony
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2024
• Subjects: Biosensors; Fluorescence sensors; FRET; Luminescence; Transition metal dichalcogenide nanosheets; FRET; Biosensors; Fluorescence sensors; Transition metal dichalcogenide nanosheets; Luminescence
• ISSN: 1010-6030; 1873-2666
• DOI: 10.1016/j.jphotochem.2024.115604

Graphical representation of fluorescence turn-on detection of BNP antigen-by-BNP specific antibody tagged gold nanoclusters – MoS2 nanosheet nano couple. [Display omitted] •An easy and prompt method for the detection of heart failure biomarker was achieved with gold nanocluster / 2D MoS2 nanosheets.•A stable fluorescence turn - on response was observed with the probe in presence of BNP in physiologically significant serum concentration levels.•The development of the probe and detection of BNP antigen can be achieved within minutes without any high-end instruments.•The probe exhibited a low detection limit of 0.165 pg/mL. In the context of elevated morbidity and mortality risk of heart failure (HF), the establishment of prompt and sensitive diagnosis of HF is vital. A nanostructured fluorescence immuno sensing probe was constructed out of noble metal nanoclusters / two-dimensional transition metal dichalcogenide nano couple to target brain natriuretic peptide (BNP) antigen, a potential multi-utility heart failure biomarker. The green fluorescence of BNP-specific monoclonal antibody-conjugated gold nanoclusters was pre-quenched by a MoS2 two-dimensional nanosheet, which is subsequently enhanced in the presence of BNP antigen via target-specific antigen–antibody interaction. The probe is free from interferences by other potentially interfering possible co-existing biomolecules and offers easy, low-cost linear detection of BNP in the concentration range 0 to 360.82 pg/mL.