Circulating CD3+56+ cell subset in pre-diabetes

• Published in: Experimental and clinical endocrinology & diabetes Vol. 122; no. 2; p. 65
• Main Authors: Dworacka, M, Wesołowska, A, Wysocka, E, Winiarska, H, Iskakova, S, Dworacki, G
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2014
• Subjects: Aged; Blood Glucose - metabolism; CD3 Complex - blood; CD56 Antigen - blood; Deoxyglucose - blood; Diabetes Mellitus, Type 2 - blood; Fasting - blood; Glycated Hemoglobin A - metabolism; Humans; Hyperglycemia - blood; Lipids - blood; Male; Middle Aged; Natural Killer T-Cells - metabolism; Natural Killer T-Cells - pathology; Plaque, Atherosclerotic - blood
• ISSN: 1439-3646
• DOI: 10.1055/s-0033-1363233

The CD3+56+ cells are a small but significant population of T lymphocytes encompassing NKT-like and NKT cells, which may play the essential role at the very early stages of atherosclerotic plaque development. The frequency and activity of CD3+56+ cells in atherosclerosis-inducing dysglycaemic disease (diabetes type 2 or pre-diabetes) is largely unknown.We analysed CD3+56+ cell count, granzyme, perforin and annexin V profiles in the peripheral blood from a group of patients with pre-diabetes, with diabetes type 2 and from non-dysglycaemic controls. Measurements were made of fasting glucose levels, HbA1c, 1,5-anhydroglucitol and lipid profile.The mean counts of CD3+56+ cells were significantly higher in patients with pre-diabetes compared to both patients with diabetes and to control group. There was an increase in the number of CD3+56+ cells producing granzyme and perforin in pre-diabetic patients compared to other groups, while there were no difference in annexin V+ populations within examined groups. It was confirmed that CD3+56+ cells count is modified by metabolic factors and their parameters, namely HbA1c and 1,5-anhydroglucitol values.It could be stated that the alterations of CD3+ 56+  cells count in peripheral blood of pre-diabetic and type 2 diabetic patients are related to different grades of carbohydrate deteriorations - postprandial hyperglycaemia and chronic hyperglycaemia.