Identification of genetic loci for powdery mildew resistance in common wheat

• Published in: Frontiers in plant science Vol. 15; p. 1443239
• Main Authors: Liu, Xia, Zhang, Xiaoqing, Meng, Xianghai, Liu, Peng, Lei, Menglin, Jin, Hui, Wang, Yanzhen, Jin, Yirong, Cui, Guoqing, Mu, Zhixin, Liu, Jindong, Jia, Xiaoyun
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 09.10.2024
• Subjects: 90K SNP array; KASP; Plant Science; powdery mildew; resistance; Triticum aestivum; KASP; powdery mildew; 90K SNP array; Triticum aestivum; resistance
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2024.1443239

Powdery mildew (PM) poses an extreme threat to wheat yields and quality. In this study, 262 recombinant inbred lines (RILs) of Doumai and Shi 4185 cross were used to map PM resistance genes across four environments. High-density genetic linkage map of the Doumai/Shi 4185 RIL population was constructed using the wheat Illumina iSelect 90K single-nucleotide polymorphism (SNP) array. In total, four stable quantitative trait loci (QTLs) for PM resistance, , , , and , were detected and explained 5.6%-15.6% of the phenotypic variances. Doumai contributed all the resistance alleles of , , , and . Among these, and overlapped with the previously reported loci, whereas and are potentially novel. In addition, six high-confidence genes encoding the NBS-LRR-like resistance protein, disease resistance protein family, and calcium/calmodulin-dependent serine/threonine-kinase were selected as the candidate genes for PM resistance. Three kompetitive allele-specific PCR (KASP) markers, for , for , and for , were developed, and their genetic effects were validated in a natural population including 100 cultivars. These findings will offer valuable QTLs and available KASP markers to enhance wheat marker-assisted breeding for PM resistance.