Plantlet Regeneration Potential from Seedling Explants of Vitegnus (Vitex agnus castus)

In this research a simple and repeatable method for regeneration of a important medicinal plant (Vitex agnus castus) described. Different seedling explants such as hypocotyl, cotyledon, root and apical meristem were cultured in MS basal media with different kinds and concentrations of PGRs. Root and...

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Bibliographic Details
Published inPakistan journal of biological sciences Vol. 10; no. 22; pp. 4113 - 4117
Main Author Chamandoos, F.
Format Journal Article
LanguageEnglish
Published Pakistan 15.11.2007
Subjects
Cotyledon - physiology
Culture Media
Models, Biological
Plant Physiological Phenomena
Plant Roots - cytology
Plant Roots - growth & development
Plant Roots - physiology
Regeneration
Seedlings - physiology
Seeds - metabolism
Temperature
Tissue Culture Techniques
Vitex - physiology
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Summary:In this research a simple and repeatable method for regeneration of a important medicinal plant (Vitex agnus castus) described. Different seedling explants such as hypocotyl, cotyledon, root and apical meristem were cultured in MS basal media with different kinds and concentrations of PGRs. Root and apical meristem explants were the only explants that have regeneration whole plantlets potential. It was interesting that regeneration whole plantlets from root and apical meristem explants have different developmental pathways. Whole plantlets from apical meristem explants regenerated by passing phase callusing whereas regeneration whole plantlets from root was direct and without phase callusing. This subject implies that we can have many manipulation possibilities in order to different objects of tissue culture by selecting different explants in vitegnus.
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ISSN:1028-8880
DOI:10.3923/pjbs.2007.4113.4117