HPLC Analysis and Antimicrobial, Antidiarrheal and Antihyperglycemic Properties of Eurya acuminata along with in silico Profiles

• Published in: Phytomedicine Plus : International journal of phytotherapy and phytopharmacology Vol. 2; no. 3; p. 100291
• Main Authors: Devnath, Hiron Saraj, Ahmed, Md. Iqbal, Medha, Maisha Maliha, Islam, Md. Naharul, Biswas, Ritu Porna, Islam, Md. Amirul, Sadhu, Samir Kumar
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2022; Elsevier
• Subjects: Antidiarrheal; Antihyperglycemic; Antimicrobial; Eurya acuminata; HPLC analysis; In silico profiling; Antimicrobial; In silico profiling; Eurya acuminata; HPLC analysis; Antihyperglycemic; Antidiarrheal
• ISSN: 2667-0313; 2667-0313
• DOI: 10.1016/j.phyplu.2022.100291

•E. acuminata is traditionally used to treat typhoid, diarrhea and sore throat.•HPLC analysis of the ethanolic leaves extract confirmed the presence of six polyphenolic compounds.•E. acuminata ethanolic leaves extract exerted antimicrobial, antidiarrheal and analgesic activities.•E. acuminata also demonstrated antihyperglycemic activity in vivo.•In silico analysis revealed better binding of the compounds. Background: Traditional usage (typhoid, diarrhea, sore throat) and previously performed studies (antioxidative and anticancer activity) have asserted that Eurya acuminata has a widespread therapeutic potential. However, most of the pharmacological aspects of this plant are yet to be explored. Methods: Broth dilution method was selected to determine the minimum inhibitory concentration (MIC) of the plant extract. Antidiarrheal activity was figured out following the castor oil-induced diarrheal inhibition method. The oral glucose tolerance test (OGTT) was performed to assess antihyperglycemic activity. Alpha-glucosidase inhibitory assay was conducted according to the supplier's method. Acetic acid-induced writhing inhibition method was used to evaluate the peripheral analgesic activity. Results: HPLC analysis detected the presence of (-)-epicatechin, p-coumaric acid, trans-ferulic acid, rosmarinic acid, myricetin, and trans-cinnamic acid. In the broth dilution method, the extract exhibited MIC of 16 μg/ml against E. coli and S. aureus and 8 μg/ml against P. aeruginosa. In castor oil-induced antidiarrheal assay, E. acuminata extract significantly reduced 44.82% and 67.24% defecation, respectively at the doses of 250 and 500 mg/kg. Furthermore, in in vivo antihyperglycemic activity, E. acuminata extract reduced blood glucose levels to 6.8 mM and 4.68 mM, at the doses of 250 and 500 mg/kg, respectively at 60 min although the extract demonstrated no α-glucosidase enzyme inhibitory activity. Apart from that, in in vivo analgesic activity, the extract inhibited writhing reflexes dose-dependently, 15.16% and 37.13% at the doses of 250 and 500 mg/kg. Conclusion: This study shows that E. acuminata might be a good source to explore antidiarrheal, antihyperglycemic, and antimicrobial compounds for new drug development. [Display omitted]