Effect of Electromagnetic Fields on Proliferation and Differentiation of Cultured Mouse Bone Marrow Mesenchymal Stem Cells

• Published in: Current medical science Vol. 25; no. 2; pp. 185 - 187
• Main Author: 吴华 任凯 赵文春 葛保剑 彭松林
• Format: Journal Article
• Language: English
• Published: China Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2005
• Subjects: Alkaline Phosphatase - metabolism; Animals; Bone Marrow Cells - cytology; Cell Differentiation; Cell Proliferation; Cells, Cultured; Cyclic AMP - metabolism; Electromagnetic Fields; Mesenchymal Stem Cells - cytology; Mice; 动物实验; 电磁场; 细胞增殖; 骨髓间质干细胞; cyclic AMP; alkaline phosphatase; electromagnetic field; marrow mesenchymal stem cells
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/bf02873572

In order to study the effects of electromagnetic fields (EMFs) on proliferation, differentiation and intercellular cyclic AMP (cAMP) in mouse bone marrow mesenchymal stem cells (MSCs) in vitro, the mouse bone MSCs were isolated and cultured in vitro. The third passage MSCs were divided into 4 groups and stimulated with EMFs. The cellular proliferation (MTT), the cellular differentiation (alkaline phosphatase activity, ALP), and the intercellular cAMP level were investigated at different time points. The results showed that EMF (50Hz pulse burst 2 mT peak) inhibited the cellular proliferation (P<0.05), enhanced the cellular differentiation (P<0.05), and increased the intercellular cAMP level (P<0.01) in the early time of the stimulation (1-3 days), but the intercellular cAMP level did not increased further in the later days. We are led to conclude that the cAMP may be involved in the mediation of the growth inhibitory and differentiation-inducing signals of specific EMFs in vitro.