An integrated microfluidic chip-mass spectrometry system for rapid antimicrobial resistance analysis of bacteria producing β-lactamases

• Published in: Chinese chemical letters Vol. 34; no. 5; p. 107790
• Main Authors: Su, Zhaochen, Hu, Wanting, Ye, Lizhen, Gao, Dan, Lin, Jin-Ming
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023
• Subjects: Antimicrobial resistance; Inhibitor; Mass spectrometry; Microfluidics; β-Lactamases; β-Lactamases; Inhibitor; Antimicrobial resistance; Mass spectrometry; Microfluidics
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2022.107790

Bacteria producing β-lactamases have become a major issue in the global public health field. To restrain the development of drug resistance and reduce the abuse of antibiotics, it is very important to rapidly identify bacteria producing β-lactamases and put forward a reasonable treatment plan. Here, an integrated microfluidic chip-mass spectrometry system was proposed for rapid screening of β-lactamase-producing bacteria and optimization of β-lactamase inhibitor dosing concentration. The concentration gradient generator followed by an array of bacterial culture chambers, as well as micro-solid-phase extraction columns was designed for sample pretreatment before mass analysis. By using the combination system, the process of the hydrolysis of antibiotics by β-lactamase-producing bacteria could be analyzed. To validate the feasibility, four antibiotics and two antibiotic inhibitors were investigated using three strains including negative control, SHV-1 and TEM-1 strains. SHV-1 and TEM-1 strains were successfully distinguished as the β-lactamase producing strains. And the acquired optimal concentrations of β-lactamase inhibitors were in accordance with the results by that obtained from the traditional microdilution broth method. The total analysis time only needed around 2 h, which was faster than conventional methods that require a few days. The technique presented herein provides an easy and rapid protocol for β-lactamase resistance related studies, which is important for the inhibition of antimicrobial resistance development and the reduction of antibiotics abuse. Here, we report a multi-channel microfluidic chip coupled with mass spectrometry to identify bacteria producing β-lactamases and carry out corresponding antimicrobial susceptibility test. This system for the analysis of bacteria producing β-lactamases is rapid, precise and high-throughput and has great potential for clinical application. [Display omitted]