MiR-133a inhibits fracture healing via targeting RUNX2/BMP2

• Published in: European review for medical and pharmacological sciences Vol. 22; no. 9; p. 2519
• Main Authors: Peng, H, Lu, S-L, Bai, Y, Fang, X, Huang, H, Zhuang, X-Q
• Format: Journal Article
• Language: English
• Published: Italy 01.05.2018
• Subjects: 3' Untranslated Regions; 3T3 Cells; Adult; Animals; Binding Sites; Bone and Bones - metabolism; Bone and Bones - physiopathology; Bone Morphogenetic Protein 2 - metabolism; Case-Control Studies; Cell Proliferation; Core Binding Factor Alpha 1 Subunit - genetics; Core Binding Factor Alpha 1 Subunit - metabolism; Female; Fracture Healing; Fractures, Bone - genetics; Fractures, Bone - metabolism; Fractures, Bone - physiopathology; Fractures, Bone - surgery; Fractures, Ununited - genetics; Fractures, Ununited - metabolism; Fractures, Ununited - physiopathology; Gene Expression Regulation; Humans; Male; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Osteoblasts - metabolism; Osteoblasts - pathology; Osteogenesis; Signal Transduction
• ISSN: 2284-0729
• DOI: 10.26355/eurrev_201805_14914

To analyze the mechanism of miR-133a in inhibiting fracture healing through regulating runt-related transcription factor 2 (RUNX2) signaling pathway. A total of 80 patients with fracture admitted to our hospital from January 2016 to January 2017 were divided into 2 groups according to nonunion fracture or healing fracture: nonunion fracture group (n = 40) and control group (n= 40). After admission, patients underwent the surgery, respectively, and the bone tissues were taken for stand-by application. The expression level of bone morphogenetic protein 2 (BMP2) was detected using the immunohistochemical method, the expression level of RUNX2 protein was detected by Western blotting, and the expression level of micro ribonucleic acid (miR)-133a was detected by quantitative polymerase chain reaction (qPCR). Moreover, the bioinformatics method was used to predict the target gene of miR-133a, and the luciferase reporter gene was used to detect the binding of miR-133a to RUNX2. Compared with those in control group, the expression of BMP2 and the relative expressions of RUNX2 protein and miR-133a were significantly decreased; the differences were statistically significant (p < 0.05). Pearson correlation analysis showed that miR-133a was negatively correlated with RUNX2. After overexpression of miR-133a, the expression level of RUNX2 was decreased, but it was increased significantly after interference in miR-133a. Besides, it was found in dual-luciferase reporter assay that miR-133a bound to RUNX2. MiR-133a inhibits the bone formation through inhibiting the RUNX2/BMP2 signaling pathway, thereby negatively regulating the fracture healing.