Evaluation of MicroRNA 145 and MicroRNA 155 as Markers of Cardiovascular Risk in Chronic Kidney Disease

• Published in: Curēus (Palo Alto, CA) Vol. 16; no. 8; p. e66494
• Main Authors: Kumar, Amit, Priyadarshini, G, Parameswaran, Sreejith, Ramesh, Ananthakrishnan, Rajappa, Medha
• Format: Journal Article
• Language: English
• Published: United States Cureus Inc 09.08.2024; Cureus
• Subjects: Blood pressure; Cardiology; Cardiovascular disease; Creatinine; Diabetes; Endothelium; Gender; Gene expression; High density lipoprotein; Kidney diseases; Lipoproteins; Mann-Whitney U test; MicroRNAs; Nephrology; Nitric oxide; Plasma; Radiology; Statistical analysis; Triglycerides; United States > US; California; micro-rna 145; chronic kidney disease; flow mediated dilatation; micro-rna 155; endothelial dysfunction
• ISSN: 2168-8184; 2168-8184
• DOI: 10.7759/cureus.66494

Background Chronic kidney disease (CKD) leads to a progressive decline in renal function, primarily due to deteriorating kidney structures. Vascular calcification is a key effect of CKD. MicroRNAs (miRNAs) play a significant role in the onset and progression of both cardiovascular illness and CKD. Aim The aim of this study was to compare biomarkers of endothelial dysfunction, 25-hydroxyvitamin D (25(OH)D), intact parathyroid hormone (iPTH), miRNA 155, and miRNA 145, in patients with CKD versus controls. Methods We recruited 60 patients with CKD and 60 controls. All participants underwent brachial artery flow-mediated dilatation (FMD). Asymmetric dimethylarginine (ADMA) levels were measured using ELISA. Levels of miRNA 145 and miRNA 155 were quantified using real-time polymerase chain reaction (PCR). Results Serum levels of miRNA 145, miRNA 155, 25(OH)D, and FMD were significantly lower in CKD patients compared to controls. Conversely, serum ADMA and iPTH levels were significantly higher in CKD patients. There was a significant negative association between miRNA 145, miRNA 155, FMD, and 25(OH)D with ADMA and iPTH. Additionally, miRNA 145, miRNA 155, FMD, and 25(OH)D showed a significant positive correlation with estimated glomerular filtration rate (eGFR) and with each other. Conclusion Lower levels of miRNA 145 and miRNA 155 and increased endothelial dysfunction correlate with CKD severity, suggesting an accelerated risk for cardiovascular disease (CVD).