Ultrastructural aspects of experimental cryptosporidiosis in pigs

The aim of this work was to determine ultrastructural changes on the intestinal mucosa and associated lymphoid tissues after an experimental infection with Cryptosporidium sp. Twelve piglets dosed orally with 1×10(6) oocysts were slaughtered on days 3, 6, 9 and 12 after inoculation. The ultrastructu...

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Published inArquivo brasileiro de medicina veterinária e zootecnia Vol. 51; no. 5; pp. 441 - 444
Main Authors Bracarense, A.P.F.R.L.(Universidade Estadual de Londrina), Reis, A.C.F.(Universidade Estadual de Londrina), Sinhorini, I.L.(Universidade de São Paulo Faculdade de Medicina Veterinária)
Format Journal Article
LanguageEnglish
Published Universidade Federal de Minas Gerais, Escola de Veterinária 01.10.1999
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Summary:The aim of this work was to determine ultrastructural changes on the intestinal mucosa and associated lymphoid tissues after an experimental infection with Cryptosporidium sp. Twelve piglets dosed orally with 1×10(6) oocysts were slaughtered on days 3, 6, 9 and 12 after inoculation. The ultrastructural lesions in the intestinal cells were irregular with thickened microvilli, cytoplasmic protrusions and vacuolation, swollen mitochondria, hypertrophic organelles and nuclei. The lymphocytes of the Peyers patches occasionally were mitotic with a larger number of lymphoblasts in the inoculated animals. O presente estudo teve por objetivo investigar as alterações ultraestruturais da mucosa intestinal e do tecido linfóide associado na inoculação experimental de Cryptosporidium sp. Doze leitões foram inoculados experimentalmente por via oral com 1×10(6) oocistos e sacrificados 3, 6, 9 e 12 dias depois. Ao exame ultraestrutural de células intestinais observou-se espessamento e irregularidade de microvilosidades, citoplasma vacuolizado e com protrusões, edema mitocondrial, hipertrofia de organelas citoplasmáticas e do núcleo. Nas placas de Peyer observou-se ocasionalmente mitose de células linfóides, verificando-se maior número de células blásticas.
Bibliography:10.1590/S0102-09351999000500008
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500008
ISSN:0102-0935
1678-4162
1678-4162
0102-0935
DOI:10.1590/S0102-09351999000500008