Tissue immunostaining of growth, pro- and anti-apoptotic biomarkers in myocardial samples from newborns with hypoxic injury

• Published in: Jornal brasileiro de patologia e medicina laboratorial Vol. 53; no. 1; pp. 46 - 52
• Main Authors: Pabis, Francisco Cesar, Simões, Mona Adalgisa, Sakiyama, Renata R., Sakiyama, Fernando Y. R, Kayano, Rafael M., Nagashima, Seigo, Noronha, Lúcia de
• Format: Journal Article
• Language: English
• Published: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia 01.01.2017; Sociedade Brasileira de Patologia Clínica
• Subjects: apoptosis; cell enlargement; cell proliferation; immunohistochemistry; MEDICAL LABORATORY TECHNOLOGY; MEDICINE, RESEARCH & EXPERIMENTAL; newborn; PATHOLOGY; proliferação de células; cell proliferation; apoptosis; hipertrofia; apoptose; newborn; immunohistochemistry; imuno-histoquímica; cell enlargement; recém-nascido
• ISSN: 1676-2444; 1678-4774; 1678-4774
• DOI: 10.5935/1676-2444.20170009

ABSTRACT Introduction: Despite the importance of cardiovascular diseases to population health, there is limited knowledge about how neonatal hypoxia, related or not with prematurity, may cause cell injury to cardiomyocytes in the neonatal period and what the consequences to adult life are. Objective: To analyze the tissue immunostaining of biomarkers involved in the process of cell death and growth in the myocardium of hypoxic newborns. Method: Human myocardium samples (left ventricle) from necropsies of hypoxic newborns were organized in multi-sample blocks and submitted to immunohistochemical reactions by the immunoperoxidase technique. The primary antibodies used were anti-B-cell lymphoma 2 (Bcl2)-associated X protein (BAX), anti-mitofusin-2 (Mfn2), anti-tumor necrosis factor receptor-associated protein 1 (TRAP1), anti-Bcl2, anti-angiotensin II, anti-serine/threonine (Akt) 1, anti-Akt2 and anti-Akt3. Tissue immunostaining data were correlated with clinical data (gender, weight, gestational age, first-minute and fifth-minute Apgar score, arterial blood pH and survival time) and pathological data (death cause and primary disease). Results: The average tissue immunostaining of BAX was 25.61%; TRAP1 was 7.86%, angiotensin II was 1.24%, Akt2 was 16.35% and Akt3 was 20.61%. The biomarkers Akt1, Mfn2 and Bcl2 presented very low or absent tissue immunostaining in most cases of this study. There was no correlation between the average biomarker tissue immunostaining and the survival time or any other clinical or pathological factor studied. Conclusion: These data seem to strengthen the coordinated action of the pro-apoptotic, anti-apoptotic and cell growth biomarkers in the myocardium of hypoxic newborns in order to determine the degree of lesion or cell death and the tissue recovery capacity.