Immunohistochemical localization of type I, III, and IV collagen in cultured human hepatic Ito (fat-storing) cells

• Published in: Kanzo Vol. 33; no. 4; pp. 327 - 330
• Main Authors: TAKAMATSU, Seigou, NAKANO, Hiroshi, NAKAGAWA, Masanori, NAKABAYASHI, Hitomi
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 1992
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.33.327

Surgically hepetectomized human hepatic specimens were cultured in vitro. Various cells, including fat-droplets containing cells, were emerged from the tissue explants. Ito cells (ICs) were separated and cultured according to the method previously reported. Type I, III and N collagens in third or fourth passage cells were detected immunohistochemically by type-specific antibodies. In the ICs, the staining reactions to type I and III collagens were positive in the form of coarse mesh-work and/or in small granules, which were predominantly located around the nucleus. Staining was also seen aligned intracellularly along cell membrane. On a type IV collagen, the staining pattern in the cells was fine mesh-work and/or in small granules, which primarily located around nucleus, but the staining reactions to type IV collagen were weak. Fibrillar staining, which diffusely distributed in the cytoplasm was detectable but weak in intensity.