Flow cytometric analysis of Kupffer cell phagocytic function in rats

• Published in: Kanzo Vol. 33; no. 4; pp. 331 - 337
• Main Authors: KATO, Kazuya, KUSANO, Mitsuo, YAMAGUCHI, Hidenori, ONODERA, Kazuhiko, MITO, Michio
• Format: Journal Article
• Language: English; Japanese
• Published: The Japan Society of Hepatology 1992
• Subjects: Flow cytometry (FCM); Phagocytic function; Reticuloendothelial system (RES)
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.33.331

New method of measurement of Kupffer cell phagocytic function in vivo by using flow cytometery (FCM) was investigated. After injection of Flurescence isothiocyanate (FITC)-latex beads into penial vein, rats were sacrificed at selected times to isolate Kupffer cells by collagenase digestion-Percoll gravity gradient centrifusion method. The reaction time increased gradually and reached a plateau at 1 hour after injection of FITC-latex beads. The most suitable concentration of FITC-latex beads solution for FCM analysis was 6×1010beads/rat. The average of FCM-phagocytic rate (FCM-PR) in normal rats was 51.4%. Most Kupffer cells phagocytized 2 beads. In reticuloendothelial system (RES) enhanced rats pretreated with OK-432, FCM-PR increased to 74.3% significantly. And also in RES depressed rats pretreated with methyl palmitate, FCM-PR was suppressed to 18.1% significantly. The correlation coefficient between FCM-PR and phagocytic index which was measured by carbon clearance method was 0.89.