Surfactant Protein-A (SP-A) Selectively Inhibits Prostaglandin F2α (PGF2α) Production in Term Decidua: Implications for the Onset of Labor
SP-A from endometrium/decidua selectively inhibits PGF2α, and the observed decrease in decidua SP-A may be critical to “decidual activation” and onset of labor at term. Context: Labor is characterized by “decidual activation” with production of inflammatory mediators. Recent data suggest that surfac...
Saved in:
Published in | The journal of clinical endocrinology and metabolism Vol. 96; no. 4; pp. E624 - E632 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chevy Chase, MD
Endocrine Society
01.04.2011
Copyright by The Endocrine Society |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | SP-A from endometrium/decidua selectively inhibits PGF2α, and the observed decrease in decidua SP-A may be critical to “decidual activation” and onset of labor at term.
Context:
Labor is characterized by “decidual activation” with production of inflammatory mediators. Recent data suggest that surfactant protein-A (SP-A) may be critical to the onset of labor in mice. Whether this is also true in humans is unclear.
Objectives:
The aim was to investigate: 1) the expression of SP-A at the maternal-fetal interface; 2) the effect of SP-A on the production of inflammatory mediators by human decidua; and 3) the association between single nucleotide polymorphisms in maternal SP-A genes and spontaneous preterm birth.
Research Design and Methods:
In situ expression of SP-A was investigated by immunohistochemistry and quantitative RT-PCR. Term decidual stromal cells were isolated, purified, and treated with/without SP-A (1–100 μg/ml), IL-1β, and/or thrombin. Levels of inflammatory mediators [IL-6, IL-8, TNFα, matrix metalloproteinase-3, monocyte chemotactic protein-1, IL-1β, PGE2, prostaglandin F2α (PGF2α)] and angiogenic factors (soluble fms-like tyrosine kinase-1, vascular endothelial growth factor) were measured in conditioned supernatant by ELISA and corrected for protein content. The effect of SP-A on eicosanoid gene expression was measured by quantitative RT-PCR.
Results:
SP-A localized to endometrium/decidua. High-dose SP-A (100 μg/ml) inhibited PGF2α by term decidual stromal cells without affecting the production of other inflammatory mediators, and this effect occurred at a posttranscriptional level. Decidual SP-A expression decreased significantly with labor. Single nucleotide polymorphisms in the SP-A genes do not appear to be associated with preterm birth.
Conclusions:
SP-A is produced by human endometrium/decidua, where it significantly and selectively inhibits PGF2α production. Its expression decreases with labor. These novel observations suggest that decidual SP-A likely plays a critical role in regulating prostaglandin production within the uterus, culminating at term in decidual activation and the onset of labor. |
---|---|
ISSN: | 0021-972X 1945-7197 |
DOI: | 10.1210/jc.2010-1496 |