Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia
To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain. A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED...
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Published in | Open access Macedonian journal of medical sciences Vol. 7; no. 10; pp. 1576 - 1581 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Macedonia
Republic of Macedonia
31.05.2019
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Subjects | |
Online Access | Get full text |
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Summary: | To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain.
A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED). Fresh, frozen paraffin-embedded archival tissue blocks were collected from Lahore General Hospital, Lahore & Oral & Maxillofacial Surgery Department of Nawaz Sharif Hospital, Yakki Gate, Lahore. The representative sections were taken and, after processing, mounted on glass slides and stained with H&E and crystal violet stains. The stained slides were then examined under an optical microscope. The efficacy of 1% crystal violet stain to identify mitotic figures in the different grades of oral epithelial dysplasia was assessed with the sample t-test. A difference of p < 0.05 was considered to be significant.
A comparison of the mitotic figure count in two categories in sections stained with both stains showed a statistically significant difference. An increase in the mean mitotic count was noted in the sections of OED stained with crystal violet in comparison to the sections of OED stained with H&E which was statistically significant (p = 0.00).
Counting of mitotic cell is the rapid and simplest way of evaluating the proliferative activity of cells. Crystal violet stain can be a rationalised step in the staining of mitotic figures compared to the usual H&E staining and can be employed as a selective stain during routine histopathological procedures. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1857-9655 1857-9655 |
DOI: | 10.3889/oamjms.2019.330 |