Transcriptome Analysis of Sputum Cells. The Modern Art of Assessing Inflammation

[...]clinical data suggest that differentiating patients with asthma into T2-high or T2-low groups is valuable in terms of predicting response to anti-inflammatory therapy with inhaled corticosteroids (5) or certain biologics (6). In the present study, sputum samples containing a mixture of differen...

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Bibliographic Details
Published inAmerican journal of respiratory and critical care medicine Vol. 199; no. 4; pp. 402 - 404
Main Authors Taube, Christian, Reuter, Sebastian
Format Journal Article
LanguageEnglish
Published United States American Thoracic Society 15.02.2019
Subjects
Asthma
Asthma - immunology
Biomarkers
Cytokines
Dendritic cells
Gastrointestinal surgery
Gene expression
Gene Expression Profiling
Humans
Inflammation
Lymphocytes
Modern art
Obesity
Patients
Sputum
Transcriptome
Weight control
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Summary:[...]clinical data suggest that differentiating patients with asthma into T2-high or T2-low groups is valuable in terms of predicting response to anti-inflammatory therapy with inhaled corticosteroids (5) or certain biologics (6). In the present study, sputum samples containing a mixture of different cell types were assessed. [...]it is difficult to determine whether changes in gene expression are associated with changes in cell composition or due to cell activation and increased gene expression. [...]sputum gene expression network analysis can be used to identify and quantify immune cells in sputum. [...]RNA-seq is a fascinating method, but remains costly, and in particular, analysis of the vast amount of data is complicated and time-consuming. [...]this approach cannot be used routinely as a diagnostic tool.
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ISSN:1073-449X
1535-4970
1535-4970
DOI:10.1164/rccm.201810-2013ED