In vitro anti-trypanosomal activities of crude extracts, β-sitosterol and α-sulphur from Buchholzia coriacea seed

The seeds of Buchholzia coriacea Engler are traditionally used as a febrifuge in Southeast Nigeria. The bibassay-guided fractionation of the crude extracts was carried out using in vitro cultures of Trypanosoma brucei brucei S427. On soxhlet extraction, methanol gave the highest yield (5.44 %) follo...

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Bibliographic Details
Published inAfrican journal of biotechnology Vol. 10; no. 69; pp. 15626 - 15632
Main Authors Nweze, N E, Anene, B M, Asuzu, I U
Format Journal Article
LanguageEnglish
Published 07.11.2011
Subjects
Buchholzia
Trypanosoma brucei brucei
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Summary:The seeds of Buchholzia coriacea Engler are traditionally used as a febrifuge in Southeast Nigeria. The bibassay-guided fractionation of the crude extracts was carried out using in vitro cultures of Trypanosoma brucei brucei S427. On soxhlet extraction, methanol gave the highest yield (5.44 %) followed by hexane (3.34%) and ethyl acetate (0.66%). The ethyl acetate extract showed the highest anti-trypanosomal activity followed by the hexane and methanol extracts. Beta-sitosterol and alpha -sulphur were isolated from B. coriacea seed extracts by preparative chromatography and identified by nuclear magnetic resonance and x-ray crystallography, respectively. Beta-sitosterol and alpha -sulphur showed modest anti-trypanosomal activities against T. b. brucei S427 in vitro with minimum inhibitory concentration (MIC) values of 12.5 and 25 mu g/ml respectively.
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ISSN:1684-5315
1684-5315
DOI:10.5897/AJB11.865