Development and evaluation of an 18F-labeled nanobody to target SARS-CoV-2's spike protein
COVID-19, caused by the SARS-CoV-2 virus, has become a global pandemic that is still present after more than two years. COVID-19 is mainly known as a respiratory disease that can cause long-term consequences referred to as long COVID. Molecular imaging of SARS-CoV-2 in COVID-19 patients would be a p...
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Published in | Frontiers in nuclear medicine Vol. 2; p. 1033697 |
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Main Authors | , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
23.11.2022
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Subjects | |
Online Access | Get full text |
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Summary: | COVID-19, caused by the SARS-CoV-2 virus, has become a global pandemic that is still present after more than two years. COVID-19 is mainly known as a respiratory disease that can cause long-term consequences referred to as long COVID. Molecular imaging of SARS-CoV-2 in COVID-19 patients would be a powerful tool for studying the pathological mechanisms and viral load in different organs, providing insights into the disease and the origin of long-term consequences and assessing the effectiveness of potential COVID-19 treatments. Current diagnostic methods used in the clinic do not allow direct imaging of SARS-CoV-2. In this work, a nanobody (NB) – a small, engineered protein derived from alpacas – and an Fc-fused NB which selectively target the SARS-CoV-2 Spike protein were developed as imaging agents for positron emission tomography (PET). We used the tetrazine ligation to
18
F-label the NB under mild conditions once the NBs were successfully modified with
trans-
cyclooctenes (TCOs). We confirmed binding to the Spike protein by SDS-PAGE. Dynamic PET scans in rats showed excretion through the liver for both constructs. Future work will evaluate
in vivo
binding to the Spike protein with our radioligands. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Sridhar Goud Nerella, National Institutes of Health (NIH), United States Laurence Carroll, Johns Hopkins University, United States Weijun Wei, Shanghai Jiao Tong University, China Edited by: Oliver Kiß, Institute for Radiopharmaceutical Cancer Research, Germany Specialty Section: This article was submitted to Radiopharmacy and Radiochemistry, a section of the journal Frontiers in Nuclear Medicine |
ISSN: | 2673-8880 2673-8880 |
DOI: | 10.3389/fnume.2022.1033697 |