Subcellular Metabolite Levels in Spinach Leaves: Regulation of Sucrose Synthesis during Diurnal Alterations in Photosynthetic Partitioning

• Published in: Plant physiology (Bethesda) Vol. 83; no. 2; pp. 399 - 407
• Main Authors: Richard Gerhardt, Stitt, Mark, Heldt, Hans W.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.02.1987
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Chloroplasts; Cytosol; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Leaves; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Phosphates; Photosynthesis; Photosynthesis, respiration. Anabolism, catabolism; Physiological regulation; Plant physiology and development; Plants; Spinach; Starches; Vacuoles; Fructose-bisphosphatase; Phosphates; Translocation; Sucrose; Enzyme; Biosynthesis; Spinacia oleracea; Sucrose-phosphate synthase; Chenopodiaceae; Vegetable crop; Dicotyledones; Angiospermae; Cellular compartment; Spermatophyta; Photosynthesis
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.83.2.399

The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.