A Study by High-resolution Two-dimensional Polyacrylamide Gel Electrophoresis of Relationships between Neisseria gonorrhoeae and Other Bacteria

• Published in: Journal of general microbiology Vol. 130; no. 12; pp. 3189 - 3201
• Main Authors: Jackson, Peter, Thornley, Margaret J, Thompson, R. J
• Format: Journal Article
• Language: English
• Published: London Soc General Microbiol 01.12.1984; New York, NY Cambridge University Press
• Subjects: Bacteriology; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Isoelectric Focusing; Microbiology; Neisseria gonorrhoeae; Neisseria gonorrhoeae - analysis; Neisseria gonorrhoeae - classification; Neisseria meningitidis; Neisseria meningitidis - analysis; Neisseria meningitidis - classification; Peptides - analysis; Systematics; Proteins; High resolution; Neisseria gonorrhoeae; Neisseriaceae; Bacteria; Branhamella catarrhalis; Identification; Neisseria meningitidis; Two dimensional electrophoresis; Soluble form; Method; Comparative study
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-130-12-3189

University of Cambridge, Department of Clinical Biochemistry, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, UK ABSTRACT Summary: High-resolution two-dimensional polyacrylamide gel electrophoresis was used to analyse the soluble proteins from seven strains of Neisseria gonorrhoeae , six strains of Neisseria meningitidis and one or two strains of twelve other species. Approximately 200 individual polypeptides could be visualized as Coomassie Blue stained spots on an electrophoretogram of N. gonorrhoeae and similar numbers were found for the other bacteria. Each species of bacterium had a distinctly different pattern of spots which could be recognized. Quantitative comparisons of 48 selected spots derived from one strain of N. gonorrhoeae with those of five other strains of gonococcus, three strains of N. meningitidis and one of Branhamella catarrhalis , showed relationships in agreement with their current taxonomic classification but with a higher level of discrimination than that of previously used methods. It was also possible to distinguish the individual gonococcal strains. It is suggested that the method could be useful for bacterial classification and identification. Present address: Division of Immunology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, UK.