Does cytosolic free Ca2+ signal neutrophil chemotaxis in response to formylated chemotactic peptide?

Cytosolic free Ca2+ concentration was measured and imaged in human neutrophils moving towards a source of formylated peptide in a micropipette held close to the cells. Under these conditions, neutrophils changed shape and displayed chemotaxis without significant or persistent global or localised ele...

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Bibliographic Details
Published inJournal of cell science Vol. 108 ( Pt 10); no. 10; pp. 3199 - 3205
Main Authors Laffafian, I, Hallett, M B
Format Journal Article
LanguageEnglish
Published England 01.10.1995
Subjects
Calcium - physiology
Cell Movement
Chemotactic Factors - metabolism
Chemotaxis
Humans
Image Processing, Computer-Assisted
Neutrophils - physiology
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Summary:Cytosolic free Ca2+ concentration was measured and imaged in human neutrophils moving towards a source of formylated peptide in a micropipette held close to the cells. Under these conditions, neutrophils changed shape and displayed chemotaxis without significant or persistent global or localised elevations in cytosolic free Ca2+. A rear-to-front persistent Ca2+ gradient of less than 0.5 nM/micron was present in the migrating neutrophils, until they reached the zone of higher peptide concentration, when an abrupt rise in cytosolic free Ca2+ concentration was triggered and chemotaxis stopped. Small localised rises in cytosolic free Ca2+, which were occasionally observed during neutrophil manoeuvring, were attributed to the effect of local deformation of the neutrophil membrane, since deformation of the membrane with a blunt micropipette caused similar Ca2+ changes. These data suggest that neutrophil chemotaxis towards a source of formylated peptide occurs without significant changes in Ca2+ signalling.
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ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.108.10.3199