Starvation–refeeding causes cellular stress responses in the gut and liver of Masu salmon Oncorhynchus masou masou

• Published in: Fisheries science Vol. 86; no. 6; pp. 1037 - 1042
• Main Authors: Kondo, Hidehiro, Sano, Hiroaki, Wang, Yuanyuan, Kawase, Junya, Shimanoki, Eiji, Jirapongpairoj, Walissara, Nozaki, Reiko, Hirono, Ikuo
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.11.2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Body organs; Body weight; Carbohydrate metabolism; Carbohydrates; Cellular stress response; Fasting; Fatty acids; Feeding; Fish; Fish & Wildlife Biology & Management; Food Science; Freshwater & Marine Ecology; Freshwater fishes; Gene expression; Genes; Glycogen; Glycogen synthase kinase 3; Glycogens; Kinases; Life Sciences; Liver; Metabolism; Nutritional status; Oncorhynchus masou; Oncorhynchus masou masou; Organs; Original Article; Protein folding; Proteins; Salmon; Starvation; Tissue; Starvation; Masu salmon; Stress; Transcriptome; Feeding
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-020-01464-8

Gene expression profiles during the transition from fasting to refeeding were investigated in the gut and liver of Masu salmon Oncorhynchus masou masou . Fish were starved for 3 days and then fed at 3% of body weight. The gut and liver were resected before and after feeding, and the tissues were used for transcriptome analyses. Twenty-nine and 45 genes were more than eightfold differently expressed in the gut and liver, respectively. Genes involved in fatty acid and carbohydrate metabolism were differentially expressed in the liver tissues. Genes involved in protein folding, such as GRP78 and endoplasmin, were significantly upregulated in both organs at 6, 12, and 24 h after feeding. Furthermore, glycogen synthase kinase (GSK) 3-binding protein, a negative regulator of GSK with pivotal roles in stress response, was downregulated in both organs 6 h after feeding. These results suggest that the change in nutritional status from fasting to refeeding is accompanied by cellular stress.