Surveillance of multidrug resistance of 6 uropathogens in a teaching hospital and in vitro control by 25 ethnomedicinal plants used by an aborigine of India

• Published in: Asian Pacific journal of tropical biomedicine Vol. 2; no. 2; pp. S818 - S829
• Main Authors: Rath, Shakti, Dubey, Debasmita, Sahu, Mahesh C, Debata, Nagen K, Padhy, Rabindra N
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2012
• Subjects: Acinetobacter; Antibacterial activity; Azadirachta; Cassia; Community infection; Hospital infection; Multidrug resistance; Plants; Pseudomonas; Terminalia; Uropathogens; Withania; Withania; Multidrug resistance; Cassia; Hospital infection; Antibacterial activity; Acinetobacter; Terminalia; Plants; Pseudomonas; Community infection; Uropathogens; Azadirachta
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/S2221-1691(12)60319-0

To evaluate antimicrobial potencies of 25 plants with reports on ethnomedicinal uses for infectious ailments by the aborigine Kandha tribe of Kalahandi district, Odisha state, India for urinary tract infections. Over a period of 6 months, multidrug resistant (MDR) strains of 6 uropathogenic bacteria Acinetobacter baumannii (A. baumannii), Citrobacter freundii (C. freundii), Klebsiella oxytoca (K. oxytoca), Proteus mirabilis (P. mirabilis), Proteus vulgaris (P. vulgaris) and Pseudomonas aeruginosa (P. aeruginosa) were isolated from clinical samples in a teaching hospital; their antibiograms were ascertained. Concentrated aqueous and ethanolic extracts of leaves and barks of plants were used for monitoring their antimicrobial potencies, by the agar-well diffusion method. Phytochemical analyses of plant parts were done. All isolated bacterial strains were resistant to 15 antibiotics of 6 groups including β-lactams. From a surveillance of bacterial isolates, it was evident that the distribution of MDR strains of each was more in hospital acquired isolates than the community acquired ones. Both aqueous and ethanolic extracts of plants, Aegle marmelos (A. marmelos), Azadirachta indica (A. indica) and Withania somnifera (W. somnifera) were highly effective against MDR isolates of all these pathogens. Several plants were moderately effective during in vitro control of the pathogens. Plants, Anthocephalus cadamba (A. cadamba), Cleistanthus collinus (C. collinus) and Oroxylum indicum (O. indicum) were totally ineffective in the control of isolated MDR uropathogen. A. indica, T. arjuna and T. alata contained the full range of phytochemicals (alkaloids, glycosides, terpenoids, reducing sugars, saponins, tannins, flavonoids and steroids), which could be attributed to the significant anti-uropathogenic activities. Plants, A. indica, A. marmelos, Cassia fistula (C. fistula), T. arjuna, Salvadora persica (S. persica), W. somnifera and Vitex negundo (V. negundo), particularly could be useful for an use as complementary/supplementary medicines for MDR uropathogens.