TM4SF19-AS1 facilitates the proliferation of lung squamous cell carcinoma by recruiting WDR5 to mediate TM4SF19

• Published in: Molecular and cellular probes Vol. 65; p. 101849
• Main Authors: Luo, Machang, Xie, Lingyan, Su, Yonghua, Zhang, Kaijun, Liang, Rongzhang, Ma, Zhiyi, Li, Youtang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2022
• Subjects: Adhesion; Lung squamous cell carcinoma; Proliferation; TM4SF19; TM4SF19-AS1; WDR5; WDR5; TM4SF19; Proliferation; TM4SF19-AS1; Adhesion; Lung squamous cell carcinoma
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1016/j.mcp.2022.101849

As reported, long non-coding RNAs are a pivotal player in lung squamous cell carcinoma (LSCC) progression. We noticed the remarkably upregulated transmembrane-4-l-six-family-19 antisense RNA 1 (TM4SF19-AS1) in LSCC and further demonstrated the function it played in LSCC and the possible molecular mechanism. Via bioinformatics approach, we evaluated TM4SF19-AS1 and TM4SF19 levels in LSCC tissue, and real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot revealed their mRNA and protein levels in LSCC cells. Cell Counting Kit-8 and colony formation assays analyzed the proliferation ability of LSCC cells, and cell adhesion ability was detected via cell adhesion assay. RNA immunoprecipitation and chromatin immunoprecipitation analyzed the underlying mechanism of TM4SF19-AS1 regulating its target, while methylation-specific PCR indicated the methylation level of TM4SF19-AS1. TM4SF19-AS1 was markedly upregulated in LSCC. Functional assays revealed that TM4SF19-AS1 could facilitate the proliferation and adhesion of LSCC. Besides, we revealed the mechanism of TM4SF19-AS1 regulation that it directly bound to WD repeat-containing protein 5 (WDR5), and was then recruited to TM4SF19 promoter region, which activated DNA demethylation, thereby suppressing malignant LSCC progression. Our research demonstrated that TM4SF19-AS1 affected LSCC cell proliferation by recruiting WDR5 to manipulate transmembrane-4-lsix-family-member-19 (TM4SF19), which offers a new observation on LSCC pathogenesis, indicating that TM4SF19-AS1 is able to be a promising target for LSCC treatment. •Upregulation of TM4SF19-AS1 facilitated LSCC proliferation and adhesion.•TM4SF19-AS1 together with WDR5 regulated TM4SF19 expression.•TM4SF19-AS1 facilitated the proliferation of LSCC by recruiting WDR5 to mediate TM4SF19.