Indirect calculation of LDL using thirteen equations in Pakistani population

• Published in: Clinica chimica acta Vol. 536; pp. 77 - 85
• Main Authors: Khan, Madeeha, Ain, Qura Tul, Nawaz, Amjad, Iqbal Khan, Mohammad, Sadiq, Fouzia
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2022
• Subjects: Friedewald equation; Homogenous assay; Lipid profile; Low density lipoprotein cholesterol; Martin equation; Teerankanchana equation; Lipid profile; Friedewald equation; Homogenous assay; Teerankanchana equation; Martin equation; Low density lipoprotein cholesterol
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/j.cca.2022.09.010

•Teerakanchana equation displayed a strong positive correlation with the measured LDL, followed by Martin equation in Pakistani population.•Teerakanchana equation performs equally well among all ranges of triglycerides.•Teerankanchana equation can be an alternative to Friedewald equation in Pakistani clinical settings. Owing to the atherogenic properties, low density lipoprotein cholesterol (LDL-C) is the primary target for treatment and diagnosis of cardiovascular diseases (CVDs), hence accurate measurement of LDL-C is critical. Despite the availability of direct measurement assays for LDL-C, it is routinely calculated by Friedewald equation in clinical settings in Pakistan mostly due to financial constraints. However, the validity of this equation is impacted by several factors, therefore several other equations have been developed for the calculation of LDL-C. LDL-C of 39,385 individuals measured directly by homogenous assays (dLDL) was compared with LDL-C calculated by thirteen equations (cLDL-C). Stratifications based on different lipids i.e., triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) were made to check the validity of these equations across all ranges of lipid profile. The correlation and median difference between dLDL and cLDL-C was statistically analyzed. Overall Teerakanchana equation displayed a strong positive correlation (ρ = 0.967) and least median difference (-8.81) with dLDL, followed by Martin equation (ρ = 0.967). For higher TG ranges (>500 mg/dL), Teerakanchana equation had the least median difference (1.31) and a strong correlation (ρ = 0.800). Our data suggest that Teerakanchana equation may be employed as an alternative to Friedewald equation for Pakistani population.