Immune regulation mediated by JAK/STAT signaling pathway in hemocytes of Pacific white shrimps, Litopenaeus vannamei stimulated by lipopolysaccharide

• Published in: Fish & shellfish immunology Vol. 130; pp. 141 - 154
• Main Authors: Yan, Peiyu, Lin, Cheng, He, Meng, Zhang, Zhuofan, Zhao, Qun, Li, Erchao
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2022
• Subjects: Immune regulatory mechanism; JAK/STAT signal pathway; Lipopolysaccharide; Litopenaeus vannamei; RNA interference; Lipopolysaccharide; JAK/STAT signal pathway; RNA interference; Litopenaeus vannamei; Immune regulatory mechanism
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2022.07.048

To understand the regulatory mechanism of Janus kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway on the immune system of the Pacific white shrimp, Litopenaeus vannamei, RNA interference technique was used to investigate the effects of JAK/STAT signaling pathway on the immune response of hemocyte in Litopenaeus vannamei stimulated by lipopolysaccharide (LPS). The results showed that 1) after 6 h of LPS stimulation, the expression levels of immune genes in hemocyte were significantly up-regulated (P < 0.05), the immune defense ability (hemocyte number, phagocytosis rate, hemagglutination activity, bacteriolytic activity, antibacterial activity, prophenoloxidase system activity) and the hemocyte antioxidant ability were significantly higher than the control group, especially at 12 h. 2) After 48 h of STAT gene interference, the expression levels of immune genes in hemocytes were significantly down-regulated, and the immune defense ability (hemocyte count, phagocytosis rate, plasma agglutination activity, lysozyme activity, antibacterial activity, proPO system activity) and the antioxidant ability were reduced and significantly lower than control. Concurrently, after LPS stimulation, the immune indexes were significantly up-regulated at 12 h to the maximum but was still lower the undisturbed LPS group. These results indicate that JAK/STAT signaling pathway is involved in the immune regulation mechanism of L. vannamei against LPS stimulation through positive regulation of cellular immune and humoral immune. These results provide a basis for further research on the role and status of JAK/STAT signaling pathway in the immune defense of crustaceans. Regulation of cellular and humoral immunity by JAK/STAT signaling pathway. [Display omitted] •After STAT RNAi, the expression level of JAK/STAT pathway genes and related immune genes down-regulated significantly.•IL-CTL could recognize LPS and bind to cell membrane receptors to activate the JAK/STAT signaling pathway.•Activated JAK/STAT signaling pathway positively regulated the hemocyte count and phagocytosis of the cellular immunity.•Activated JAK/STAT signaling pathway positively regulated the antibacterial activity and other humoral immune functions.