Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling

• Published in: Biosensors & bioelectronics Vol. 47; pp. 520 - 523
• Main Authors: Xu, Hui, Xu, Pingping, Gao, Shanmin, Zhang, Shengxiao, Zhao, Xingchun, Fan, Chunhai, Zuo, Xiaolei
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.09.2013; Elsevier
• Subjects: Biological and medical sciences; Biosensor; Biosensors; Biotechnology; DNA recycling; DNAzyme; Fluorescence; Fundamental and applied biological sciences. Psychology; Lead; Methods. Procedures. Technologies; Various methods and equipments; DNAzyme; Fluorescence; Lead; DNA recycling; Biosensor; DNA; Recycling; Recognition
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2013.03.062

Here, we have demonstrated an ultra-high sensitive detection platform with the detection limit of 5pM for an environmental toxin—Pb2+. We designed a Pb2+ triggered exonuclease aided DNA recycling system to improve the detection sensitivity. In our system, a Pb2+ dependent 8–17 DNAzyme and its substrate were used to form hybridization duplex. In the presence of Pb2+, the substrate was cleaved and disassociated from the duplex. Then, the released 8–17 DNAzyme was used as a target of the exonuclease aided DNA recycling system which can amplify the fluorescence signal by recycling the 8–17 DNAzyme continuously. Then, the sensitive Pb2+ detection are accomplished and the detection limit of Pb2+ was down to 5pM which is about 1000 times lower than the traditional detection method based on the 8–17 DNAzyme. •An ultrahigh sensitive Pb2+ detection platform with the detection limit of 5pM was developed (1000 times lower than traditional DNAzyme method).•Combines the DNAzyme and exnuclease aided target recycling system•Using DNA amplification to detect non-nucleic acids targets.•The Pb2+ detection platform is simple and does not need expensive instrumentations