Defatting and Clarification of Protein Hydrolysates by Using Chitosan Solutions

The possibility of the use of small amounts of chitosan for defatting and clarification of protein solutions prepared by enzymatic hydrolysis was tested. The following treatment conditions were shown to be optimal: a chitosan concentration range, from 1.0 to 1.5 gram per kilogram raw weight; pH of t...

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Published inApplied biochemistry and microbiology Vol. 37; no. 6; pp. 629 - 634
Main Authors Novikov, V Yu, Mukhin, V A
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.11.2001
Subjects
Biochemistry
Carbohydrates
Enzymes
Proteins
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Summary:The possibility of the use of small amounts of chitosan for defatting and clarification of protein solutions prepared by enzymatic hydrolysis was tested. The following treatment conditions were shown to be optimal: a chitosan concentration range, from 1.0 to 1.5 gram per kilogram raw weight; pH of the precipitation medium from 8.0 to 8.5; and duration of the incubation of the protein hydrolysate solution with chitosan, less than 1 h. The hydrolysate defatting grade was found to depend on the degree of chitosan deacetylation. A possible mechanism of the chitosan-induced effects was suggested. The use of chitosan allows the mass fraction of enzyme protein hydrolysates to be reduced fourfold to fivefold.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-6838
1608-3024
DOI:10.1023/A:1012367520667