Characterization of the RDC1 gene which encodes the canine omolog of a proposed human VIP receptor Expression does not correlate with an increase in VIP binding sites

• Published in: FEBS letters Vol. 300; no. 2; pp. 149 - 152
• Main Authors: Cook, Jonathan S., Wolsing, Dana Hance, Lameh, Jelveh, Olson, Christy A., Correa, Paul E., Sadee, Wolfgang, Blumenthal, Edward M., Rosenbaum, Jan S.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.03.1992
• Subjects: 0.01 M NaH2PO4, pH 7.1, 0.1 mM EDTA; 1×SSPE, 0.18 M NaCl; bovine serum albumin; BSA; DMEM; Dulbecco's modified Eagle medium; FBS; fetal bovine serum; fMLP; G-protein; IL-8; interleukin-8; N-formyl-Met-Leu-Phe; RDC1; Receptor cloning; Receptor expression; vasoactive intestinal peptide; VIP; VIP receptor; wild-type (untransfected) cells; Receptor cloning; fMLP; RDC1; Receptor expression; VIP receptor; IL-8; 1×SSPE, 0.18 M NaCl; DMEM; BSA; FBS; G-protein; VIP; WT
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80184-I

We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5′ untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP receptor when expressed in CHO-K1 and COS-7 cells (Sreedharan, S.P. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 4986–4990). Northern analysis indicates that CHO-K1 cells endogenously express a 2.1 kb RDC1 mRNA. However, while CHO-K1 cells possess detectable low affinity [125I]VIP binding sites, VIP binding is not altered in membranes of CHO-K1 cells expressing varying amounts of the RDC1 gene construct. Further, endogenous VIP binding is not increased by transient expression of RDC1 in COS-7 cells. Taken together, the data suggest that RDC1 is not a canine homolog of the proposed VIP receptor.