Potent trophic activity of spermidine supramolecular complexes in in vitro models

AIM:To test the growth-promoting activity of the polyamine spermidine bound to various polymeric compounds in supramolecular complexes.METHODS:A thiazolyl blue cell viability assay was used to determine the growth-promoting potency of spermidine-supramolecular complexes in a human skin fibroblast ce...

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Published inWorld journal of biological chemistry Vol. 4; no. 3; pp. 71 - 78
Main Authors Ghisalberti, Carlo A, Morisetti, Alberto, Bestetti, Alessandro, Cairo, Gaetano
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Co., Limited 26.08.2013
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Summary:AIM:To test the growth-promoting activity of the polyamine spermidine bound to various polymeric compounds in supramolecular complexes.METHODS:A thiazolyl blue cell viability assay was used to determine the growth-promoting potency of spermidine-supramolecular complexes in a human skin fibroblast cell line exposed to spermidine and different spermidine-supramolecular complexes that were obtained by combining spermidine and polyanionic polymers or cyclodextrin.Reconstituted human vaginal epithelium was exposed to a specific spermidinesupramolecular complex,i.e.,spermidine-hyaluronan(HA)50,and cell proliferation was determined by Ki-67immunohistochemical detection.Transepithelial electrical resistance and histological analysis were also performed on reconstituted human vaginal epithelium to assess tissue integrity.RESULTS:The effect of spermidine and spermidinesupramolecular complexes was first tested in skin fi-broblasts.Spermidine displayed a reverse dose-related mode of activity with mmol/L growth inhibition,whereas 30%stimulation over basal levels was detected at mol/L and nmol/L levels.Novel spermidine-supramolecular complexes that formed between spermidine and polyanionic polymers,such as HA,alginate,and polymaleate,were then tested at variable spermidine concentrations and a fixed polymer level(0.1%w/v).Spermidine-supramolecular complexes stimulated the cell growth rate throughout the entire concentration range with maximal potency(up to 80%)at sub-mol/L levels.Similar results were obtained with spermidine-(-cyclodextrin),another type of spermidine-supramolecular complex.Moreover,the increased expression of Ki-67 in the reconstituted human vaginal epithelium exposed to spermidine-HA 50 showed that the mode of action behind the spermidine-supramolecular complexes was increased cell proliferation.Functional and morphological assessments of reconstituted human vaginal epithelium integrity did not show significant alterations after exposure to spermidine-HA,thus supporting its safety.CONCLUSION:Spermidine found in spermidine-supramolecular complexes displayed potentiated regenerative effects.Safety data on reconstituted human vaginal epithelium suggested that assessing spermidinesupramolecular complex efficacy in atrophic disorders is justified.
Bibliography:Carlo A Ghisalberti;Alberto Morisetti;Alessandro Bestetti;Gaetano Cairo;Department of Biomedical Sciences for Health, University of Milan
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Author contributions: Ghisalberti CA conceived experiments, acquired and analyzed data, and wrote the manuscript; Morisetti A acquired and analyzed data and co-wrote the manuscript; Bestetti A acquired and analyzed data and helped finalize the paper; and Cairo G analyzed data and wrote the manuscript.
Telephone: +39-2-50315338 Fax: +39-2-50315338
Correspondence to: Gaetano Cairo, Professor, Department of Biomedical Science for Health, University of Milano, Via Mangiagalli 31, 20133 Milano, Italy. gaetano.cairo@unimi.it
ISSN:1949-8454
1949-8454
DOI:10.4331/wjbc.v4.i3.71