Potent trophic activity of spermidine supramolecular complexes in in vitro models
AIM:To test the growth-promoting activity of the polyamine spermidine bound to various polymeric compounds in supramolecular complexes.METHODS:A thiazolyl blue cell viability assay was used to determine the growth-promoting potency of spermidine-supramolecular complexes in a human skin fibroblast ce...
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Published in | World journal of biological chemistry Vol. 4; no. 3; pp. 71 - 78 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Baishideng Publishing Group Co., Limited
26.08.2013
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Subjects | |
Online Access | Get full text |
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Summary: | AIM:To test the growth-promoting activity of the polyamine spermidine bound to various polymeric compounds in supramolecular complexes.METHODS:A thiazolyl blue cell viability assay was used to determine the growth-promoting potency of spermidine-supramolecular complexes in a human skin fibroblast cell line exposed to spermidine and different spermidine-supramolecular complexes that were obtained by combining spermidine and polyanionic polymers or cyclodextrin.Reconstituted human vaginal epithelium was exposed to a specific spermidinesupramolecular complex,i.e.,spermidine-hyaluronan(HA)50,and cell proliferation was determined by Ki-67immunohistochemical detection.Transepithelial electrical resistance and histological analysis were also performed on reconstituted human vaginal epithelium to assess tissue integrity.RESULTS:The effect of spermidine and spermidinesupramolecular complexes was first tested in skin fi-broblasts.Spermidine displayed a reverse dose-related mode of activity with mmol/L growth inhibition,whereas 30%stimulation over basal levels was detected at mol/L and nmol/L levels.Novel spermidine-supramolecular complexes that formed between spermidine and polyanionic polymers,such as HA,alginate,and polymaleate,were then tested at variable spermidine concentrations and a fixed polymer level(0.1%w/v).Spermidine-supramolecular complexes stimulated the cell growth rate throughout the entire concentration range with maximal potency(up to 80%)at sub-mol/L levels.Similar results were obtained with spermidine-(-cyclodextrin),another type of spermidine-supramolecular complex.Moreover,the increased expression of Ki-67 in the reconstituted human vaginal epithelium exposed to spermidine-HA 50 showed that the mode of action behind the spermidine-supramolecular complexes was increased cell proliferation.Functional and morphological assessments of reconstituted human vaginal epithelium integrity did not show significant alterations after exposure to spermidine-HA,thus supporting its safety.CONCLUSION:Spermidine found in spermidine-supramolecular complexes displayed potentiated regenerative effects.Safety data on reconstituted human vaginal epithelium suggested that assessing spermidinesupramolecular complex efficacy in atrophic disorders is justified. |
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Bibliography: | Carlo A Ghisalberti;Alberto Morisetti;Alessandro Bestetti;Gaetano Cairo;Department of Biomedical Sciences for Health, University of Milan ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: Ghisalberti CA conceived experiments, acquired and analyzed data, and wrote the manuscript; Morisetti A acquired and analyzed data and co-wrote the manuscript; Bestetti A acquired and analyzed data and helped finalize the paper; and Cairo G analyzed data and wrote the manuscript. Telephone: +39-2-50315338 Fax: +39-2-50315338 Correspondence to: Gaetano Cairo, Professor, Department of Biomedical Science for Health, University of Milano, Via Mangiagalli 31, 20133 Milano, Italy. gaetano.cairo@unimi.it |
ISSN: | 1949-8454 1949-8454 |
DOI: | 10.4331/wjbc.v4.i3.71 |