Effect of dilution on viability of vitrified-warmed mouse embryos

The present study was conducted to establish a suitable dilution method at room temperature for mouse 8-cell embryos (CD-1) vitrified by VS3 solution. Viability of the zygotes vitrified by VS1 and VS3 solutions was also examined. Superovulated 8-cell embryos and zygotes were equilibrated with VS3 or...

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Published inKachiku hanshokugaku zasshi (Tōkyō. 1977) Vol. 35; no. 4; pp. 211 - 216
Main Authors Kono, T. (Tokyo Univ. of Agriculture (Japan). Nodai Research Inst.), Kwon, O.Y, Ichinoe, K, Nakahara, T
Format Journal Article
LanguageEnglish
Japanese
Published THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 1989
Subjects
BEBES
CONSERVACION DE EMBRIONES
CONSERVATION D'EMBRYON
DILUTION METHOD
EMBRYO PRESERVATION
EMBRYO TRANSFER
ENFANT EN BAS AGE
INFANTS
METHODE
METHODS
METODOS
MICE
MOUSE EMBRYO
RATON
SOURIS
TRANSFER TEST
TRANSFERENCIA DE EMBRIONES
TRANSFERT EMBRYONNAIRE
VIABILIDAD
VIABILITE
VIABILITY
VITRIFICATION
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Summary:The present study was conducted to establish a suitable dilution method at room temperature for mouse 8-cell embryos (CD-1) vitrified by VS3 solution. Viability of the zygotes vitrified by VS1 and VS3 solutions was also examined. Superovulated 8-cell embryos and zygotes were equilibrated with VS3 or VS1 solutions and plunged into LN2, . After warming, the vitrified-warmed embryos were diluted with various pro-cedures and cultured in vitro. The blastocysts obtained were transferred to pseudo-pregnant recipients. The high viability of vitrified-warmed mouse 8-cell embryos was obtained when the embryos were diluted with 0.5 M sucrose-HB1 solution for 5 min or with 1.5 M sucrose-HB1 and 0.5 M sucrose-HB1 solution for 5 min each at room tempera-ture; 90% and 87% of the embryos were recovered as morphologically normal after dilution, and 89% and 86% of these developed to blastocyst in vitro, respectively. After transfer to recipients, 60% and 59% of the blastocysts developed to full term. When the zygotes were vitrified by VS3 solution, the survived zygotes was few after dilution and culture in vitro. In contrast, 20% of vitrified and warmed zygotes by VS1 solution was developed to blastocysts, and live young were obtained after transfer. In conclusion, VS3 solution is effective for cryopreservation of mouse 8-cell embryos by vitrification, but not for the zygotes.
Bibliography:9300358
L53
ISSN:0385-9932
DOI:10.1262/jrd1977.35.211