Measurement of Critical Osmotic Pressure of Biological Cells : Minimum Cell-to-Volume Ratio as an Estimation of Viability

The critical osmotic pressure that can be endured by biological cells is measured. Optimal parameters of the freezing process as well as the prefreezing process are restricted by the critical osmotic pressure, which can be translated into the critical normalized volume of the cell. Viability of the...

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Bibliographic Details
Published inJSME International Journal Series B Fluids and Thermal Engineering Vol. 42; no. 3; pp. 561 - 565
Main Authors SHIRAKASHI, Ryo, TANASAWA, Ichiro
Format Journal Article
LanguageEnglish
Published The Japan Society of Mechanical Engineers 1999
Subjects
Biological Engineering
Biological Tissue
Cell Membrane Permeability
Cryopreservation
Cryoprotective Agent
Medical Engineering
Prefreezing Process
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Summary:The critical osmotic pressure that can be endured by biological cells is measured. Optimal parameters of the freezing process as well as the prefreezing process are restricted by the critical osmotic pressure, which can be translated into the critical normalized volume of the cell. Viability of the cells is measured after the cells are exposed to a certain osmotic pressure, and the viability can be estimated from the viewpoint of biophysical functions. In this study, endothelial cells and smooth muscle cells comprising porcine arteries are selected as samples. The viability of endothelial cells is estimated by a dye-exclusion test, and that of smooth muscle cells is estimated by measuring the contraction-relaxation rate. Optimal parameters of the freezing process as well as the prefreezing process can be obtained by estimating the normalized volume of the cell. The critical normalized volume of cells can be estimated from the measured critical osmotic pressure.
ISSN:1340-8054
1347-5371
DOI:10.1299/jsmeb.42.561