Combined Effects of the C161T and Pro12Ala PPARγ2 Gene Variants with Insulin Resistance on Metabolic Syndrome: A Case–Control Study of a Central Tunisian Population

• Published in: Journal of molecular neuroscience Vol. 52; no. 4; pp. 487 - 492
• Main Authors: Youssef, Sarraj Mohamed, Mohamed, Najah, Afef, Slimani, Khaldoun, Ben Hamda, Fadoua, Neffati, Fadhel, Najjar Mohamed, Naceur, Slimene Mohamed
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.04.2014
• Subjects: Adult; Amino Acid Substitution; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Cell Biology; Female; Genetic Predisposition to Disease - epidemiology; Genetic Predisposition to Disease - genetics; Genetic Variation; Genotype; Humans; Insulin Resistance - genetics; Male; Metabolic Syndrome - epidemiology; Metabolic Syndrome - genetics; Middle Aged; Neurochemistry; Neurology; Neurosciences; Polymorphism, Restriction Fragment Length; PPAR gamma - genetics; Prevalence; Proteomics; Risk Factors; Tunisia - epidemiology; Metabolic syndrome; Insulin resistance; PPARγ2 polymorphism
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-013-0161-y

We investigated the synergism between variants at the PPARγ locus (C161T and Pro12Ala polymorphisms) with insulin resistance on metabolic syndrome (MS). Five hundred twenty-two subjects were investigated for biochemical and anthropometric measurements. The diagnosis of MS was based on the IDF definition (2009). The HOMA 2 was used to determine HOMA-β, HOMA-S, and HOMA-IR from FPG and FPI concentrations. PCR-RFLP was performed for DNA genotyping. We showed that carriers of the Pro/Pro had a significantly higher FPG, FPI, and HOMA-IR. In addition, Pro/Pro subjects also display reduced HOMA-β and HOMA-S together compared to X/Ala (Pro/Ala and Ala/Ala) subjects. Furthermore, subjects with C/C have a significantly lower FPG, FPI, and HOMA-IR and higher HOMA-S compared to X/T (C/T and T/T) subjects. The C/C genotype carriers with an Ala allele group had significantly reduced FPG, FPI, HOMA-IR, and TG and elevated HOMA-S and HOMA-β than the different genotype combinations. We suggest that the haplotype composed of the C/C genotype carriers with an Ala allele of PPARγ2 group enhances susceptibility to the MS in a central Tunisian population.