Albumin gene expression during mouse odontogenesis

Albumin protein is present in developing teeth of several species. Oligomer primers and cRNA probes specific for albumin were designed to perform RT-PCR, and for in situ hybridization, respectively. In situ hybridization failed to reveal albumin expression in any tooth cells, however, albumin PCR pr...

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Bibliographic Details
Published inAdvances in dental research Vol. 10; no. 2; p. 119
Main Authors Yuan, Z A, McAndrew, K S, Collier, P M, Koyama, E, Chen, E, Sandgren, E P, Gibson, C W
Format Journal Article
LanguageEnglish
Published United States 01.11.1996
Subjects
Albumins - biosynthesis
Albumins - genetics
Animals
Cell Line
Dental Enamel - metabolism
Gene Expression
Mice
Mice, Inbred Strains - genetics
Mice, Transgenic - genetics
Molar - cytology
Molar - metabolism
Odontogenesis - physiology
Odontogenic Tumors - metabolism
Odontogenic Tumors - pathology
Periodontal Ligament - cytology
Periodontal Ligament - metabolism
RNA, Messenger - analysis
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Summary:Albumin protein is present in developing teeth of several species. Oligomer primers and cRNA probes specific for albumin were designed to perform RT-PCR, and for in situ hybridization, respectively. In situ hybridization failed to reveal albumin expression in any tooth cells, however, albumin PCR products were amplified from tissues adhering to the roots of developing teeth from four-week-old mice. It is concluded that this source is not the primary source of albumin protein found in developing enamel, because of the location and level of expression of albumin mRNA in periodontal tissue.
ISSN:0895-9374
DOI:10.1177/08959374960100020301