Changes in antioxidant enzyme activities in detached leaves of cucumber exposed to chilling
We studied changes in biochemical and physiological status, level of oxidative damage, and antioxidant enzyme activities in detached leaves of cucumber plants (Cucumis sativus L. cv. Pyunggangnaebyungsamchuk) that were exposed to a low temperature (4°C). Chlorophyll fluorescence (Fv/Fm) declined dur...
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Published in | Journal of plant biology = Singmul Hakhoe chi Vol. 47; no. 2; pp. 117 - 123 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
Springer Nature B.V
30.06.2004
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Subjects | |
Online Access | Get full text |
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Summary: | We studied changes in biochemical and physiological status, level of oxidative damage, and antioxidant enzyme activities in detached leaves of cucumber plants (Cucumis sativus L. cv. Pyunggangnaebyungsamchuk) that were exposed to a low temperature (4°C). Chlorophyll fluorescence (Fv/Fm) declined during the chilling treatment, but was slowly restored after the tissues were returned to 25°C. Likewise, the fluorescence quenching coefficient and relative water content decreased during the stress period, but then increased during recovery. In contrast, we detected a significant rise in protein and hydrogen peroxide contents in the chilled leaves, as well as higher activities for superoxide dismutase, ascorbate peroxidase, peroxidase, and glutathione reductase. However, the level of catalase decreased not only during chilling but also after 24 h of recovery. These results indicate that exposure to low temperatures acts as an oxidative stress. Moreover, we propose that a regulating mechanism exists in the detached cucumber leaves and contains an antioxidant defense system that induces active oxygen species, thereby alleviating the effects of chilling stress within 12 h. |
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ISSN: | 1226-9239 1867-0725 |
DOI: | 10.1007/BF03030641 |